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Vector Laboratories
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Addgene inc
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Addgene inc
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Genechem
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Thermo Fisher
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Addgene inc
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Image Search Results
Journal: Bioscience Reports
Article Title: Interaction with IP6K1 supports pyrophosphorylation of substrate proteins by the inositol pyrophosphate 5-InsP7
doi: 10.1042/bsr20240792
Figure Lengend Snippet: Figure 2. IP6K1 binds pyrophosphorylation substrate proteins and CK2 (A–C) Co-precipitation of endogenous NOLC1 (A), TCOF (B) or UBF1 (C) with IP6K1. N-terminally SFB-tagged IP6K1 was expressed in HEK293T cells, pulled down with streptavidin-sepharose beads, and probed to detect endogenous NOLC1, TCOF or UBF1. (D)
Article Snippet: Human IP6K1 cDNA (GenBank ID NM 001242829.2) and catalytically inactive IP6K1 (K226A) mutant were subcloned in pCDNA3.1 as previously described [22]. cDNA encoding human IP6K1 domains, i.e., FL, N-lobe, C-lobe, IDR-1, IDR-2, PDKG motif with IP helices, and IDR-1 with C-lobe, were cloned into the
Techniques: